3% glucose 0.088V

impt procedure: add everything in 1st

THEN add in salt bridge. SALT BRIDGE LAST TO GO IN

there are some radical changes to our proj again. hopefully mit soon, so that we can discuss.

cathode solution possibly change to potassium ferrycyanide K3Fe(CN)6

in the broth side, we'll add 1ml 50micromolar methylene blue. methylene blue acts as electron carrier for the oxidation, it helps carry the electrons to our anode so the transfer is many times more efficient, leading to better readings of voltage. hehe.

change: have to restart all data collection.

we are changing from e. coli MM294 to strain K12. fml. but nvm. shldn't take more than 2.5 weeks to generate this result.

found a way to add the catalyst. according to boss, can use methylene blue in broth side.

den we analyse kinetics. shld b quite scientifically sound.

but main analysis is still on glu conc, catalyst conc, fruit peel extract conc.

detail:

bacteria cell conc:

absorbance read at 600nm, blank of nb. check absorbance of bacteria in broth for 1.000 against blank, if not adjust accordingly

3% glu nb
0.061V stable. repeating next mon

change: carbon rod fully submerged in bacterial broth.

1% glu, nb 1l, CuSO4.
stable: 0.060V as of 24h

3% glu, nb, bacteria 60ml, CuSO4 1%
0.045V stable.

making broth:

1. weigh in mass of nb for 1l
2. weigh in % of glu
3. put in 1000ml water
4. autoclave
5. 50ml bacteria

bacteria broth making:

1. 50ml broth
2. ? loops of bacteria
3. incubate at 30degC overnight

cathode solution: bacteria. electrode: carbon rod
anode solution: copper(II) sulfate. electrode: copper piece.

1st run:
5% glu, nb, bacteria 1l, CuSO4 1%.
0.306V max stable.

2nd run:
7% glu, nb, bacteria 1l, CuSO4 1%
0.045V stable.